Dataset features


Application: Gene expression microarray analysis
Number of samples: 37
Release date: Feb 9 2009
Last update date: Mar 20 2012
Access: Public
Diseases: Fibrocystic Breast Disease, Tumor Lysis Syndrome, Cardiomyopathy, Hypertrophic, Familial
Chemicals: Estrogens, Progestins
Dataset link Gene expression profiles of progestin-induced canine mammary hyperplasia and spontaneous mammary tumors

Experimental Protocol

Gene expression changes in three different canine mammary tissues types, namely normal, progesterone-induced hyperplasia (CMH) and spontaneous tumors (CMC), were analyzed by comparing to a common reference. Total RNA was isolated from the canine mammary tissues used. In vitro transcription followed by labeling and hybridization to cDNA microarray was carried out. In a reference design of hybridization, labeled cRNA from each sample was hybridized against reference pooled cRNA. Out of 37 tissues samples used in this study, 8 were normal, 8 were CMH and 21 were CMC. Equal numbers of samples per tissue group were labeled with Cy3 and Cy5 (dye swap). Reference cRNA was prepared by adding an equal quantity of cRNA derived from individual samples used in this study. Statistical analysis of the log-transformed normalized data was done using SAM (significance analysis of microarray) and differentially expressed genes in CMH and CMC compared to normal tissue were identified.








Nagesha Rao