Computational protocol: Lipidomic profiling of high fat diet induced obesity in mice: importance of cytochrome P450 derived fatty acid epoxides

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Protocol publication

[…] All data are expressed as the mean ± standard error of the mean (SEM). For the comparison between the control group and HFD group, Shapiro-Wilk test was used to verify the normality of data. When data were normally distributed, statistical significance was determined using two-side t-test; otherwise, significance was determined by Mann-Whitney U test. All of these data analysis was performed by using SigmaPlot software (San Jose, CA). The principal component analysis (PCA) was implemented using MetaboAnalyst ( The data were scaled using auto scaling before the analysis. P values less than 0.05 are reported as statistically significant. […]

Pipeline specifications

Software tools SigmaPlot, MetaboAnalyst
Application Miscellaneous
Organisms Mus musculus
Chemicals Fatty Acids, Unsaturated