|Application:||Gene expression microarray analysis|
|Number of samples:||9|
|Release date:||Apr 13 2010|
|Last update date:||Aug 28 2018|
|Dataset link||Differential gene expression patterns in lung carcinogenesis mediated by loss of mouse tumor supressor Gprc5a|
Gprc5a wild type cells (WT-NLE) and Gprc5a knockout cells (NULL-NLE) were isolated and cultured from trachea of three week old Gprc5a wild type and knockout mice, respectively. MDA-F471 mouse lung adenocarcinoma cells were derived de novo from an adenocarcinoma that was found in the lung of a female Gprc5a-knockout mouse sacrificed 16 months after NNK tobacco carcinogen i.p. injection based on a protocol approved by the M.D. Anderson Cancer Center Institutional Animal Care and Use Committee. Following RNA extraction and purification, the transcriptome of the Gprc5a wild type and knockout cells were analyzed by microarray analysis using the Affymetrix MG-430 2.0 murine array platform.