Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis, BS-seq analysis
Number of samples: 31
Release date: Dec 14 2017
Last update date: Nov 27 2018
Access: Public
Chemicals: Cytosine, Nucleotides
Dataset link Paternal easiRNAs establish the triploid block in Arabidopsis

Experimental Protocol


15 small RNA sequencing libraries, 8 genome-wide cytosine methylation libraries and 8 RNA-seq libraries. 7 small RNA libraries were generated from mature pollen grains of diploid and tetraploid Col-0 wt and nrpd1a mutants (1n and 2n pollen, respectively) and Col-0 wt and the mutant backgrounds dcl4 rdr6 and rdr6. 8 small RNA libraries were generated from seeds at 6 days after pollination from the cross of maternal Ler with paternal Col-0 wt and the mutant backgrounds osd1,nrpd1a, and nrpd1a osd1. 8 cytosine methylation libraries were generated from hand-dissected endosperm at 6-7 days after pollination from the cross of maternal Ler with paternal Col-0 wt and the mutant backgrounds osd1,nrpd1a, and nrpd1a osd1. 8 RNA-seq libraries were generated from hand-dissected endosperm at 6-7 days after pollination from the cross of maternal Ler with paternal Col-0 wt and the mutant backgrounds osd1,nrpd1a, and nrpd1a osd1.

Repositories


GEO

GSE84122

ENA

SRP078043

BioProject

PRJNA328101

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Contact


Juan Santos-González