Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 3
Release date: Mar 25 2016
Last update date: Mar 25 2016
Access: Public
Chemicals: Carbon, Nitrogen
Dataset link Organic Nitrogen Source is Critical for Welan Production in Sphingomonas sp. ATCC 31555

Experimental Protocol


Sphingomonas sp. ATCC 31555 strain (stored in our laboratory) was first seeded in an inoculum medium (20 g/L glucose, 3 g/L yeast extract, 3 g/L malt extract, and 5 g/L fish meal protein peptone, pH 7.0), and then cultured in a fermentation medium containing 40 g/L sucrose, 4.0 g/L nitrogen source, 0.6 g/L KH2PO4, and 0.2 g/L MgSO4.7H2O at 37°C. The nitrogen sources used in the present study were as follows: NaNO3 (4.0 g/L) as inorganic nitrogen (IN), beef extract (4.0 g/L) as organic nitrogen (ON), and NaNO3 (1.5 g/L) + beef extract (2.5 g/L) as complex nitrogen (CN). All cultivations were conducted in flasks with constant rotary shaking at 400–1,000 rpm and 37°C.

Repositories


GEO

GSE79497

ArrayExpress

E-GEOD-79497

ENA

SRP072322

BioProject

PRJNA316326

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Contact


xiaopeng Xu