Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jul 1 2013
Last update date: Aug 23 2018
Access: Public
Taxon: Mus musculus
Computational protocol: affy, limma, GOstats, Primer-BLAST, GEO
Dataset link Expression data after irradiating mMSCs for 2 hours with broadband terahertz source

Experimental Protocol


Growing in petri dish mouse mesenchymal stem cell cultures are irradiated for 2 hours with pulsed ultrafast (35 fs) broadband (centered at ~10 THz) source. Three independent biological experiments were conducted. In each experiment, a control mMSC culture was placed adjacent to the irradiated sample, and the temperature was kept at 26-27ºC for both the control and the irradiated sample. Immediately after completing the irradiation, total RNA was extracted from the irradiated sample and the control, and microarrays were used to identify differential changes in gene expression between the irradiated sample and its respective control. In each experiment, the mMSC cultures were synchronized to be at the same differentiation time point immediately before the irradiation.

Repositories


GEO

GSE41083

ArrayExpress

E-GEOD-41083

BioProject

PRJNA175819

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Contact


Boian Alexandrov
Boian S Alexandrov

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