Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Sep 4 2014
Last update date: Jan 25 2018
Access: Public
Chemicals: Morphine
Dataset link Whole-genome expression profile in zebrafish embryos after chronic exposure to morphine

Experimental Protocol


Zebrafish embryos were divided into two experimental groups: control embryos and embryos at 5 hpf exposed to 10 nM morphine and collected at 24 hpf (covering the complete embryogenesis). Morphine was administered to the embryos in their water environment, i.e., diluted in E3 embryonic medium. The exposition to begun at the stage of 5 hpf (end of blastula) is continuous, in order to study the chronic effects of the exposure to drug. Microarray experiments were performed using six replicates for each condition, which contained the RNA of approximately one hundred embryos to minimize the influence of potential individual differences between the animals and technical variation introduced by tissue preparation. We previously reported that a concentration of 10 nM morphine is the highest concentration that can be used without a toxic effect on the embryos, and close to 5% of the morphine diluted in the E3 medium is detected in the embryo.

Repositories


GEO

GSE61062

ArrayExpress

E-GEOD-61062

BioProject

PRJNA260174

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Contact


Raquel Rodriguez
Raquel E. Rodriguez

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