Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 38
Release date: Mar 1 2015
Last update date: May 13 2016
Access: Public
Diseases: Breast Neoplasms, Carcinoma, Ductal, Breast
Dataset link Deregulated microRNAs in triple-negative breast cancer revealed by deep sequencing

Experimental Protocol


In the present study, a panel of 24 triple-negative breast cancer and 14 adjacent normal tissue samples were examined for the presence of deregulated miRNA genes using the high-throughput sequencing technology. Total RNA was extracted from the triple-negative breast cancer and adjacent normal samples for preparation of small RNA libraries. Each small RNA library was constructed from total RNA of each sample using the SOLiD Total RNA-Seq Kit (Applied Biosystems, Foster City, CA, USA). Upon completion of polymerase chain reaction (PCR) amplification, small RNA libraries were purified using the SOLiD Library Micro Column Purification Kit (Applied Biosystems) and hybridized to the template beads using the SOLiD EZ bead system (Applied Biosystems). The template beads were amplified and deposited onto subtract for ligation sequencing by SOLiD 4 System (Applied Biosystems).

Repositories


GEO

GSE40049

ArrayExpress

E-GEOD-40049

ENA

SRP014829

BioProject

PRJNA172756

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