Computational protocol: A Label-Free and Ultrasensitive Immunosensor for Detection of Human Chorionic Gonadotrophin Based on Graphene FETs

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Protocol publication

[…] Raman spectra was collected in an XploRA Raman system (Horiba, Middlesex, UK) running a LabSpec and equipped with a 532 nm HeNe laser delivering 20 mW of laser power at the sample. An Olympus BX41 microscope (Olympus Corp., Tokyo, Japan) with a 100 µm slit width, a 300 µm confocal hole, 1200 T grating and 1000× magnification was used. An MPlan N 100× microscope objective (N.A.: 0.90, W.D.: 0.21, F.N.: 22) focused the laser on the sample into a spot of ~7.45 µm diameter. A thermoelectrically cooled charge coupled device (CCD) camera was used for detection. Single spectra were collected for bare, coated and non-coated dentine by graphene from 1100 cm−1 to 3000 cm−1 range with five accumulations each lasting 15 s.X-ray photoelectron spectroscopy (XPS) was performed with Kratos AXISULTRA with a mono-chromated Al kα X-ray source (1486.6 eV) using an emission current of 10 mA and an anode potential of 12 kV (120 W). The ULTRA was used in fixed analyzer transmission (FAT) mode, with pass energy of 80 eV for wide scans and pass energy 20 eV for high resolution scans. Cyclic Voltammetry (CV) measurements were carried out in 10 mM ferricyanide aqueous solution (1 M KCl solution) at room temperature with a scan potential range from 0.8 V to −0.4 V and a scan rate of 100 mV·s−1. [...] A Keysight B1500A semiconductor device parameter analyzer with an MPS150 probe station (Cascade Microtech GmbH, Thiendorf, Germany) was employed to investigate the electrical characteristics and sensing performance of the FET device at room temperature. The transistor measurement on the sensor was carried out by measuring the drain current (Ids) as a function of the gate voltage (Vg) (−100 V to +100 V) with a fixed drain-source voltage (Vds = 100 mV). The sensing signal of the device was recorded by monitoring the Ids as a function of drain-source voltage (Vd) (−1 V to +1 V) with a fixed gate voltage (Vds = 0 V). The data were analysed using SigmaPlot 13 software (Systat Software Inc, London, UK). […]

Pipeline specifications

Software tools LabSpec, SigmaPlot
Databases VDS
Applications Miscellaneous, NMR-based metabolomics
Organisms Homo sapiens
Chemicals Graphite