Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 4
Release date: Jun 1 2014
Last update date: Oct 16 2017
Access: Public
Genes: RBP4
Taxon: Saccharomyces cerevisiae
Dataset link Coordinating Expression of RNA Binding Proteins with Their mRNA Targets

Experimental Protocol


Both the PUF3 deletion strain and wild type BY4742 (MATa, his3Δ1, leu2Δ0, met15Δ0, ura3Δ0) strain were cultured overnight in YPD media. 0.01 OD cells were transferred into fresh YPEG media until 1.0 OD. Total RNA was extracted by the standard Trizol protocol. mRNA was then purified using oligo-dT DynaBeads. cDNA sequencing library was constructed according to the protocol described by Wang et al. After sequencing, the reads were also mapped into S. cerevisiae genome by SOAP2 with no more than two mismatches. RPKM was used to represent the expression level of each gene.

Repositories


GEO

GSE55419

ArrayExpress

E-GEOD-55419

ENA

SRP039016

BioProject

PRJNA239549

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Contact


Huifeng Jiang

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