Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Oct 29 2008
Last update date: Jan 18 2013
Access: Public
Diseases: Heart Diseases
Dataset link Differential gene expression analysis in the larval heart of Drosophila

Experimental Protocol


In the present study, we described two distinct whole-genome microarray experiments to identify differentially expressed genes in the drosophila larval heart (or dorsal vessel) : 1) comparison of the transcriptome of heart (posterior part) versus aorta (anterior part) from the third instar larval cardiac organ, 2) comparison of the transcriptome of the entire larval dorsal vessel to that of sibling whole larvae. For each microarray experiment, 4 biological replicates were used through a dye-switch (or biological dye-swap) microarray experimental design in which they were labelled so that each of two conditions has half the samples tagged with Cy3 dyes (2 with Cy3) and the other half with Cy5 dyes (2 with Cy5), then independently hybridized in a reverse and balanced way on dual-channel microarrays (4 arrays). A graphical representation of the design used on this study is showed in the associated manuscript.

Repositories


GEO

GSE12539

BioProject

PRJNA112895

Download


Contact


Bruno ZEITOUNI
Maria Tevy