Computational protocol: Small‐molecule Hedgehog inhibitor attenuates the leukemia‐initiation potential of acute myeloid leukemia cells

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Protocol publication

[…] Bone marrow cells from PF‐913‐treated mice (n = 3) and vehicle‐treated mice (n = 3) were separated with the MACS human CD45 MicroBeads Kit (Miltenyi Biotec, Tokyo, Japan) into AML cells, and total RNA was purified. DNA microarray analysis was undertaken by Filgen (Nagoya, Japan) using a CodeLink Human Whole Genome Bioarray (Applied Microarrays, Tempe, AZ, USA). The Gene Set Enrichment Analysis (GSEA) algorithm was used to evaluate the statistical significance of gene expression. Gene Set Enrichment Analysis is supported by the Broad Institute (http://www.broadinstitute.org/gsea/index.jsp). [...] Data are presented as the means ± SEM. The statistical significance of differences between groups was determined using Student's t‐test. Comparisons among more than two groups were analyzed with the Tukey–Kramer test followed by one‐way anova. Statistical analyses were carried out with JMP pro software (SAS Institute, Cary, NC, USA). […]

Pipeline specifications

Software tools GSEA, JMP Pro
Applications Miscellaneous, Gene expression microarray analysis
Organisms Mus musculus, Homo sapiens
Diseases Leukemia, Neoplasms, Leukemia, Myeloid, Acute
Chemicals Cytarabine