Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Feb 14 2011
Last update date: Mar 22 2012
Access: Public
Chemicals: Copper
Dataset link Global transcriptional response to copper of Enterococcus faecalis wild-type and copY mutant

Experimental Protocol


RNA was extracted from four different culture conditions: 1) untreated WT cells; 2) Cu-treated WT cells; 3) untreated copy mutant cells; and 4) Cu-treated copy mutant cells. Each array contained 72,000 oligonucleotides 60-mer, with an average of 11 oligos designed for the 3,114 open reading frames of E. faecalis V583, that represent twice the genome of the bacterium (two technical replicates per arrays). For wild type and copY mutant four independent hybridizations (two biological replicates per treatment paired with their respective controls) were performed. Gene expression data from WT cells was compared to Cu-treated WT cells; and data from copY mutant cells were compared to Cu-treated copY mutant cells.

Repositories


GEO

GSE20453

BioProject

PRJNA125113

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Contact


Mauricio Latorre