Dataset features

Specifications


Application: ATAC-seq analysis
Number of samples: 4
Release date: Nov 21 2018
Last update date: Nov 30 2018
Access: Public
Genes: IL2, FOXP3, CD4, PPA1, ERRa
Taxon: Mus musculus
Dataset link Role of interleukin 2 on chromatin landscapes in lymph node Foxp3+ CD4+ regulatory (Treg) cells

Experimental Protocol


LNs from 6-8 weeks old aged-matched females either Il2ramut/mut (2 mice) or WT (2 mice) Foxp3-RFP+ reporter mice were harvested and RFP+ Treg cells were enriched by negative selection (dynabeads) and flow-sorted (Aria III) before proceeding to extraction of nuclei, transposition and library preparation for sequencing (ATAC-seq).

Repositories


GEO

GSE103193

ENA

SRP116329

BioProject

PRJNA400471

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Contact


Gregoire Lauvau