Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 27
Release date: Feb 2 2018
Last update date: Nov 28 2018
Access: Public
Diseases: Neoplasms, Rhabdomyosarcoma
Computational protocol: HTSeq, limma, Enrichr, Partek Genomics Suite, GOrilla, PCGP
Dataset link HNRNPH1 is required for rhabdomyosarcoma cell growth and survival

Experimental Protocol


Two individual siRNAs targeting HNRNPH1 were used to knockdown HNRNPH1 in RD, RH30, and RH41 cells. Both siRNAs were done in triplicate in all cell lines. Briefly, 20nM siRNA was transfected with RNAiMax in 6-well dishes (2 × 105 RD and RH30 or 2.4 × 105 RH41) for 12 hours, after which media was changed and cells collected 48 hours post transfection.

Repositories


GEO

GSE104559

ENA

SRP119331

BioProject

PRJNA413052

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Contact


David Finkelstein

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