Computational protocol: Electricity generation from digitally printed cyanobacteria

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Protocol publication

[…] The printed electrode circuit pattern was designed using graphic design software Adobe Illustrator (Adobe Systems, USA). Firstly, CNTs were printed in five over-layers to prepare a bespoke electrode circuit on paper, with anode and cathode electrodes with a size ratio of 1:2. Secondly Synechocystis cells were printed in five overlays onto the printed anodic areas (Fig. ). The same inkjet printer, HP Deskjet 340, was used to print the electrode circuit, using the multiwall carbon nanotubes (MWCNTs) inkjet ink, Nink-1000: multiwall (NanoLab, USA), and the photosynthetic cells, Synechocystis PCC 6803 or WT-G. BPV printed modules consisted of one zigzag anode and one zigzag cathode with surface areas 1.36 cm2 and 2.73 cm2 respectively (Fig. ). For the stability experiments two printed BPV modules with the zigzag pattern were connected in series. The freshly printed cells on the BPV modules were transferred onto a BG-11 agar plate within an hour of printing and were incubated at 30 °C under continuous illumination of 10 W m−2 s−1 from white fluorescent lamps for 3 to 4 days. […]

Pipeline specifications

Software tools Adobe Illustrator, SHELX
Applications Miscellaneous, Protein structure analysis
Chemicals Carbon