|Application:||Gene expression microarray analysis|
|Number of samples:||17|
|Release date:||May 31 2010|
|Last update date:||Jul 31 2017|
|Chemicals:||Biotin, Carbon, Glucose|
|Dataset link||Gene expression underlying the effects of biotin deficiency in rat liver, worms and yeast|
Five male rats (Wistar strain) aged 21 days and 50g of weight were made biotin-deficient, and another five rats of similar gender, age and weight served as controls. Nematodes synchronized in L1 stage grown in minimal medium (MM) with 5 and 0.05 nM of biotin (sufficient and deficient, respectively). Biotin-deficient cultures of yeast were obtained by growing cells overnight in a defined synthetic minimal (SD) media with dextrose as the principal carbon source (2%) and (NH4)2SO4 as nitrogen source with aminoacids, minerals and vitamins as complements but without biotin. Cells were centrifuged, washed, and used to inoculate cultures in MMY with 0.4nM and 8nM of biotin adjusted to an A600 absorbance of 0.04 by triplicate. Cells were harvested when cultures reached an A600 absorbance of 0.6-0.8.
Pablo Antonio Velázquez-Arellano