|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Oct 9 2013|
|Last update date:||Aug 13 2018|
|Diseases:||Cardiovascular Diseases, Neoplasms|
|Dataset link||Age-related changes in microRNA levels in serum [mRNA]|
HeLa cells were transfected with 20 nM biotinylated miRIDIAN miR-1248 mimic (Thermo Scientific) or a negative control, biotinylated miRIDIAN C. elegans miR-67 (here named Con-miR) using Lipofectamine 2000. Forty eight hours post transfection, total RNA was isolated using the mirVana kit (Invitrogen) according to manufacturer’hree replicate experiments were performed and a technical repeat was included for each sample. Samples were analyzed using Illumina's Sentrix Human HT-12 ver4 Expression BeadChips (Illumina, San Diego, CA).
Kevin G Becker