Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Oct 9 2013
Last update date: Aug 13 2018
Access: Public
Diseases: Cardiovascular Diseases, Neoplasms
Dataset link Age-related changes in microRNA levels in serum [mRNA]

Experimental Protocol


HeLa cells were transfected with 20 nM biotinylated miRIDIAN miR-1248 mimic (Thermo Scientific) or a negative control, biotinylated miRIDIAN C. elegans miR-67 (here named Con-miR) using Lipofectamine 2000. Forty eight hours post transfection, total RNA was isolated using the mirVana kit (Invitrogen) according to manufacturer’hree replicate experiments were performed and a technical repeat was included for each sample. Samples were analyzed using Illumina's Sentrix Human HT-12 ver4 Expression BeadChips (Illumina, San Diego, CA).

Repositories


GEO

GSE51309

ArrayExpress

E-GEOD-51309

BioProject

PRJNA222388

Download


Contact


Kevin Becker
Kevin G Becker