Dataset features

Specifications


Dataset type: Expression profiling by array
Number of samples: 6
Release date: Feb 15 2019
Last update date: Feb 16 2019
Access: Public
Dataset link Ectopic expression of DNA methyltransferases DNMT3A2 and DNMT3L leads to aberrant hypermethylation and postnatal lethality in mice

Experimental Protocol


We generated transgenic mice expressing both DNMT3A2 and DNMT3L ectopically in broad somatic cells throughout the lifetime (DNMTs-Tg mice). All DNMTs-Tg mice showed delayed postnatal growth and finally died within 20 weeks after birth. We hypothesized that the exogenous DNMT3A2 and DNMT3L abnormally methylated some genes to produce these severe phenotypes. As DNA methylation generally silences gene expression, we conducted comprehensive gene expression analyses in heart tissues of DNMTs-Tg mice to identify genes that were abnormally hypermethylated by exogenous DNMT3A2 and DNMT3L.

Repositories


GEO

GSE126566

BioProject

PRJNA522392

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Contact


Keisuke Sasaki