|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Jul 19 2010|
|Last update date:||May 11 2017|
|Diseases:||Bone Marrow Diseases|
|Dataset link||Expression Data from BALB/c and Stat6-deficient bone marrow derived macrophages (BMDM)|
Macrophages were differentiated from bone marrow cells in supplemented RPMI 1640 for 7 days in the presence of 10% supernatant from the M-CSF producing fibroblast cell line L929. After removal of nonadherent cells, macrophages were detached using 5mM EDTA treatment, washed, counted, and replated at a density of 10x6 cells/mL. Macrophages were cultured for 24 hours in medium (control) or in the presence of IL-4 (10mg/mL). F4/80 positive macrophages were sorted with a purity of 99% and RNA was isolated (Trizol/Chloroform) according to the manufacturer's instructions. RNA was labeled (biotin), fragmented, and hybridized to the arrays according to the manufacturer's recommendations.