Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Jul 19 2010
Last update date: May 11 2017
Access: Public
Diseases: Bone Marrow Diseases
Dataset link Expression Data from BALB/c and Stat6-deficient bone marrow derived macrophages (BMDM)

Experimental Protocol


Macrophages were differentiated from bone marrow cells in supplemented RPMI 1640 for 7 days in the presence of 10% supernatant from the M-CSF producing fibroblast cell line L929. After removal of nonadherent cells, macrophages were detached using 5mM EDTA treatment, washed, counted, and replated at a density of 10x6 cells/mL. Macrophages were cultured for 24 hours in medium (control) or in the presence of IL-4 (10mg/mL). F4/80 positive macrophages were sorted with a purity of 99% and RNA was isolated (Trizol/Chloroform) according to the manufacturer's instructions. RNA was labeled (biotin), fragmented, and hybridized to the arrays according to the manufacturer's recommendations.

Repositories


GEO

GSE20030

ArrayExpress

E-GEOD-20030

BioProject

PRJNA124115

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Contact


Silke Huber