|Application:||Gene expression microarray analysis|
|Number of samples:||27|
|Release date:||Feb 9 2017|
|Last update date:||Jan 9 2018|
|Genes:||NELFCD, IFNG, TNF, IL4, IL10, CD274|
|Dataset link||Interferon-g and Tumor Necrosis Factor-a Polarize Bone Marrow Stromal Cells Uniformly to a Th1 Phenotype|
BMSCs (passage 4) were stimulated with 6.5 or 65 ng/ml of IFN-g (R&D System Minneapolis, MN, USA), and with 1.5 or 15 ng/ml of TNF-a (R&D System Minneapolis, MN, USA), or the combination of IFN-g and TNF-a (1.5 ng/ml of TNF-a + 6.5 ng/ml of IFN-g or 15 ng/ml of TNF-a + 65 ng/ml of IFN-g). After the adherent BMSCs were incubated with the cytokines for 48 hours, the BMSCs were harvested using trypsin and they were then evaluated by gene expression profiling.
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