Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 27
Release date: Feb 9 2017
Last update date: Jan 9 2018
Access: Public
Diseases: Neoplasms
Genes: NELFCD, IFNG, TNF, IL4, IL10, CD274
Dataset link Interferon-g and Tumor Necrosis Factor-a Polarize Bone Marrow Stromal Cells Uniformly to a Th1 Phenotype

Experimental Protocol


BMSCs (passage 4) were stimulated with 6.5 or 65 ng/ml of IFN-g (R&D System Minneapolis, MN, USA), and with 1.5 or 15 ng/ml of TNF-a (R&D System Minneapolis, MN, USA), or the combination of IFN-g and TNF-a (1.5 ng/ml of TNF-a + 6.5 ng/ml of IFN-g or 15 ng/ml of TNF-a + 65 ng/ml of IFN-g). After the adherent BMSCs were incubated with the cytokines for 48 hours, the BMSCs were harvested using trypsin and they were then evaluated by gene expression profiling.

Repositories


GEO

GSE77814

BioProject

PRJNA311612

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Contact


PING JIN

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