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Specifications


Application: miRNA array analysis
Number of samples: 15
Release date: Feb 14 2017
Last update date: Feb 14 2017
Access: Public
Diseases: Diabetes Mellitus
Chemicals: Metformin
Dataset link Metformin Induces Dicer via Regulation of the RNA-binding protein AUF1

Experimental Protocol


Groups of one-year old male B6C57/J mice were maintained on ad libitum AIN-93G SD diet, a 40% caloric restriction of the AIN-93G SD diet, or an ad libitum AIN-93G diet supplemented with 0.1% w/w metformin for the rest of their lives. Five mice from each of these 3 groups were selected and total RNA including miRNAs was isolated from the liver using the Absolutely RNA miRNA Kit (Agilent). Quality and quantity of the samples was checked with the Agilent 2100 bioanalyzer using RNA 6000 Nano chips. The Agilent miRNA Complete Labeling and Hyb Kit was used to generate fluorescently-labeled miRNA. This method involves the ligation of one Cyanine 3-pCp molecule to the 3' end of the RNA molecules. Arrays were hybridized to Agilent Mouse miRNA Microarray Release 15.0, 8x15K arrays using the entire labeled RNA product at 55 degrees C for at least 20 hours. The arrays were washed, and the bound miRNA was scanned in the Agilent scanner at 5um resolution. The data was extracted using Agilent's Feature Extractor Version 11.5.1.1, and this data was analyzed after z-score normalization. The natural log of all scores were used to find the avg and std of each array and the z-score normalization was calculated. Z-score = (raw value - avg)/std

Repositories


GEO

GSE73393

BioProject

PRJNA296876

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Contact


Kevin Becker

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