Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Mar 31 2013
Last update date: Aug 15 2018
Access: Public
Chemicals: Nitrates, Nitrites
Dataset link Root nitrate response of Col-0 plants and tga1/tga4 mutant plants

Experimental Protocol


Arabidopsis seedlings of the Col-0 and tga1/tga4 genotypes were grown on hydroponic medium containing 1X MS salts without Nitrogen, supplemented with 0.5 mM ammonium succinate as Nitrogen source and 3 mM sucrose on a Percival chamber under a photoperiod of 16 hours of light (100 μE/m2/sec) and 8 hours of dark at 22°C for 14 days. The plants were treated at the onset of the light cycle with 5 mM KNO3 or 5 mM KCl as control for 2 hours. Whole roots were cut from seedlings and frozen on liquid Nitrogen. Total RNA was extracted using the TriZol reagent. 3 independent biological replicates were performed.

Repositories


GEO

GSE43011

ArrayExpress

E-GEOD-43011

BioProject

PRJNA184053

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Contact


Jose Alvarez
Jose Miguel Alvarez