Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 95
Release date: Jul 28 2014
Last update date: Jul 28 2014
Access: Public
Diseases: Communicable Diseases, Hepatitis, Influenza, Human, Tumor Virus Infections
Genes: IFNA1
Dataset link Differential Responses of Plasmacytoid Dendritic Cells to Influenza and Distinct Viral Pathogens

Experimental Protocol


pDCs from 3 separate donors (biological replicates) were stimulated with PBS (control 1) (n=3), conditioned medium (control 2) (n=3), or HCV JFH-1 at 1.00x10^4 (n=3), 1.00x10^5 (n=3), or 1.00x10^6 (n=3) RNA copies/ml. pDCs from 3 separate donors (biological replicates) were stimulated with PBS (control) (n=3) or influenza virus A/PR/8/34 (H1N1; Charles River Laboratories) at 4 (n=3), 32 (n=3), 256 (n=3), or 2,048 (n=3) HAU/ml. pDCs from 3 separate donors (biological replicates) were stimulated with PBS (control) (n=3) or HIV-1 IIIB (Advanced Biotechnologies) at 1.00x10^2 (n=3), 1.00x10^3 (n=3), 1.00x10^4 (n=3), or 1.00x10^5 (n=3) 50% tissue culture infective doses (TCID50)/ml. pDCs from 10 separate donors (biological replicates) were treated with PBS (control) (n=10), HCV at 1.00x10^6 RNA copies/ml (n=10), HIV-1 BaL at 1.00x10^5 TCID50/ml (Advanced Biotechnologies) (n=10), HIV-1 IIIB at 1.00x10^5 TCID50/ml (Advanced Biotechnologies) (n=10), or influenza virus A/PR/8/34 (H1N1; Charles River Laboratories) at 4 HAU/ml (n=10).

Repositories


GEO

GSE59837

BioProject

PRJNA256332

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Contact


Francesco Marincola