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Specifications


Application: RNA-seq analysis
Number of samples: 20
Release date: May 19 2017
Last update date: Nov 20 2017
Access: Public
Chemicals: Monosaccharides
Dataset link The transcription factor PDR-1 is a multi-functional regulator and key component of pectin deconstruction and catabolism in Neurospora crassa

Experimental Protocol


Data was gathered from carbon source transfer experiments; N. crassa WT and Δpdr-1 mutant were pre-grown for 16 hrs on sucrose, washed 3 times in media without carbon-source and then transferred to either sucrose (WT only), L-rhamnose, pectin or no carbon source, the samples were induced for additional 4 hrs; all samples were performed in biological triplicates except WT on pectin, which was performed in duplicates; sequencing was perfomed on the Illumina HiSeq2000

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Nils Thieme

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