Computational protocol: Tool-use practice induces changes in intrinsic functional connectivity of parietal areas

Similar protocols

Protocol publication

[…] Group ICA was performed on week 0 and 8 rsfMRI data separately using the Group ICA of fMRI Toolbox (GIFT v1.3i). We extracted 20 group-independent components, only 1 of which was selected as a component of interest (sensory motor network), based on its spatial distribution in the brain, covering the primary sensory area (S1), primary motor area (M1), and the supplementary motor area (SMA) (Beckmann et al., ; De Luca et al., ). Individual resting state sensory motor network (rsSMN) components extracted from group ICA were first converted into z-score maps. Then, we used these pairs of rsSMN z-score maps as an input for the two-sample paired t-test (week 0 vs. week 8, p < 0.001, size > 8 voxels). As a control of task-selectivity in resting state networks, we compared the default mode networks (DMN) of weeks 0 and 8 to determine whether motor practice affected other networks. We selected one component which centers on the precuneus and posterior cingulate cortex, and additionally covers medial prefrontal cortex and lateral parietal areas, as DMN (Raichle et al., ). Then, the same procedure was applied to DMN for the second level comparison. [...] For this, we analyzed task fMRI data of week 0 and week 8 altogether collectively. The task fMRI data were analyzed using FMRI Expert Analysis Tool (FEAT) version 5.98, which is a part of the FMRIB Software Library (FSL). We performed GLM analysis for every individual data of week 0 and 8. Then we did group analysis to find common activation areas by chopsticks-handling task both before and after practice; 8 subjects × 2 times, total 16 data set. Z statistic images were thresholded using clusters determined by Z values > 4.0 and a (corrected) cluster significance threshold of P = 0.05 (Worsley, ). By doing this, we could make one group-averaged task activation map for chopsticks-handling. We selected tool-use-related brain regions based on our task activation results and rsSMN, and then drew 5 × 5 × 5 cubic regions of interest (ROIs) in these regions manually. Additionally, we compared the patterns of task activation during chopsticks-handling before and after practice of 8 weeks with the same threshold as above. […]

Pipeline specifications

Software tools GIFT, FSL
Application Functional magnetic resonance imaging
Organisms Homo sapiens