Computational protocol: Two Figla homologues have disparate functions during sex differentiation in half-smooth tongue sole (Cynoglossus semilaevis)

Similar protocols

Protocol publication

[…] The 5′- and 3′-RACE fragments were assembled using DNASTAR software (DNASTAR, Madison, WI) to obtain the full-length cDNA sequences of Figla_tv1 and Figla_tv2. Sequence identity and similarity of the two translated Figla proteins from C. semilaevis with known Figla proteins from other vertebrates were assessed using the MegAlign program (DNASTAR) with Clustal W method. Sequence alignments of two Figla nucleotide sequences were generated using the Clustal X and GeneDoc programs. Multiple sequence alignments of Figla proteins from different species were conducted using the Clustal X and edited by BOXSHADE (http://www. ch.embnet.org/software/BOX_form.html). […]

Pipeline specifications

Software tools SIAS, Clustal W, BoxShade
Databases Full Length cDNA
Application Proteome data visualization
Organisms Cynoglossus semilaevis
Chemicals Amino Acids, Steroids