|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Dec 21 2010|
|Last update date:||Aug 23 2018|
|Dataset link||Gene expression profile of mature plasmacytoid dendritic cells (PDC) after the deletion of transcription factor E2-2|
Inducible deletion of E2-2 (Tcf4) has been performed by administering tamoxifen to conditional E2-2flox/flox Rosa26-CreER+ mice or to E2-2flox/flox Rosa26-CreER- control littermates. Four or six days later total splenocytes were isolated, pooled from 2-3 mice and PDC (CD11b- B220+ CD11clow Bst2+) were isolated by sorting. Global gene expression profiles of E2-2-deficient (null) and control (Ctrl) PDC were compared using Affymetrix microarrays (GPL1261).