|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Oct 22 2010|
|Last update date:||Jul 26 2018|
|Dataset link||Expression data of cHL cell lines after KLF4 activation|
KM-H2-KLF-ER and L428-KLF-ER cells were incubated with 200 µM 4-OHT or vehicle (ethanol). The experiment was done twice. After 24 h, total RNA was isolated with RNeasy mini kit (QIAGEN). Microarray analyses were performed using 200 ng total RNA as starting material and 5.5 µg ssDNA per hybridization (GeneChip Fluidics Station 450; Affymetrix, Santa Clara, CA). The total RNAs were amplified and labeled following the Whole Transcript (WT) Sense Target Labeling Assay (http://www.affymetrix.com). Labeled ssDNA was hybridized to Human Gene 1.0 ST Affymetrix GeneChip arrays (Affymetrix, Santa Clara, CA). The chips were scanned with a Affymetrix GeneChip Scanner 3000 and subsequent images analyzed using Affymetrix® Expression Console Software (Affymetrix). Probe level data were obtained using the robust multichip average (RMA) normalization algorithm.