Computational protocol: Angiotensin II Overstimulation Leads to an Increased Susceptibility to Dilated Cardiomyopathy and Higher Mortality in Female Mice

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Protocol publication

[…] Ventricular myocytes were preincubated with 10 μmol/L Fluo-4AM and Ca2+ sparks on quiescent ventricular myocytes were acquired using a Zeiss LSM 710 laser scanning microscope in line scan mode. Ca2+ sparks parameters were measured and quantified using the ImageJ Sparkmaster plugin. […]

Pipeline specifications

Software tools ImageJ, SparkMaster
Applications Laser scanning microscopy, Microscopic phenotype analysis
Organisms Homo sapiens, Mus musculus
Chemicals Angiotensin II