Computational protocol: Comparative analyses and structural insights of the novel cytochrome P450 fusion protein family CYP5619 in Oomycetes

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Protocol publication

[…] P450 motifs EXXR and CXG sequence logos were generated as described elsewhere,. Briefly, CYP5619 family members were aligned using ClustalW multiple alignment using MEGA7. After sequence alignment the EXXR and CXG region amino acids (4 and 16 amino acids respectively), were selected and entered in the WebLogo program (http://weblogo.berkeley.edu/logo.cgi). As a selection parameter, the image format was selected as PNG (bitmap) at 300 dpi resolution. The generated EXXR and CXG logos were used for analysis and compared to the different P450 family EXXR and CXG logos that have been published and are available to the public,. […]

Pipeline specifications

Software tools Clustal W, MEGA, WebLogo
Application Genome data visualization
Organisms Danio rerio, Saprolegnia diclina
Chemicals Amino Acids, Fatty Acids, Heme, Hydrogen, Linoleic Acid