Computational protocol: The Signature Sequence Region of the Human Drug Transporter Organic Anion Transporting Polypeptide 1B1 Is Important for Protein Surface Expression

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[…] The topology of OATP1B1 was predicted using the online prediction programs TOPCONS [], Phobius [], TMpred [], TopPred II [], Membrain [], PHDhtm [], Split 4.0 [], Philius [], HMMTOP [], SVMtm [], ConPred II [], SOSUI [], TMHMM [], TSeg [], PRED-TMR [], and MEMSAT3 []. A homology model was generated using the Protein Homology/analogY Recognition Engine V 2.0 (PHYRE2) [], which modeled the OATP1B1 sequence on the known structure of GlpT []. [...] Statistical significance of data was calculated using a two-tailed unpaired Student's t-test (Graphpad Prism 5, CA USA) and statistical significance of normalized data was calculated using a two-tailed one sample t-test (Sigmaplot 11.0, Systat Software, CA, USA), both with P < 0.05 as significant. […]

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