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Base calling is the process by which raw data from the sequencing instrument is converted to nucleotide sequences. This is performed by base calling software that is usually run from the instrument itself. As we have seen, sequencing instruments generate raw data in different forms (fluorescence intensity, electrical impulse, etc.), and therefore base calling is specific to the particular platform being used.
(Duncan et al., 2017) Next-Generation Sequencing in the Clinical Laboratory. Diagnostic Molecular Pathology.