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Protocols

BaseSpace specifications

Information


Unique identifier OMICS_01213
Name BaseSpace
Alternative names Consensus assessment of sequence and variation, CASAVA
Software type Framework/Library
Interface Command line interface
Restrictions to use License purchase required
Biological technology Illumina
Operating system Unix/Linux
Parallelization MapReduce
License Commercial
Computer skills Advanced
Stability Stable
High performance computing Yes
Registration required Yes
Maintained Yes

Subtools


  • 16S Metagenomics
  • Amplicon DS
  • Bacterial Analysis Pipeline
  • Cancer Variant Caller
  • ChiPSeq
  • DNA Amplicon app
  • E. coli Serotyping App
  • Enrichment
  • GENIUS Metagenomics
  • Hap.py Benchmarking
  • ITS Metagenomics
  • Melanome Profiler

Versioning


No version available

BaseSpace citations

 (1505)
call_split

FACS Seq analysis of Pax3 derived cells identifies non myogenic lineages in the embryonic forelimb

2018
Sci Rep
PMCID: 5956100
PMID: 29769607
DOI: 10.1038/s41598-018-25998-1
call_split See protocol

[…] d, corresponding to six (E11.5), seven (E12.5), nine (E13.5), and six (E14.5) biological replicates. Primary Illumina data image analysis, base calling, and read-quality filtering were done using the Casava pipeline version 1.8.2 (Illumina). Each sample was processed and analyzed with the same methods. After filtering low quality reads TopHat version 2.1.0 was used to align all reads to the mm10 g […]

call_split

Case Report: Identification of an HNF1B p.Arg527Gln mutation in a Maltese patient with atypical early onset diabetes and diabetic nephropathy

2018
PMCID: 5952643
PMID: 29764441
DOI: 10.1186/s12902-018-0257-z
call_split See protocol

[…] Image analysis was performed with the default parameters of Illumina RTA pipeline, and base calling was carried out using CASAVA. The sequence reads were mapped and aligned to the Human Reference Genome (UCSC hg19, NCBI build 37) using the Burrows-Wheeler transformation algorithm, and duplicated reads were removed using […]

call_split

Transcriptome analysis of alcohol treated microglia reveals downregulation of beta amyloid phagocytosis

2018
PMCID: 5952855
PMID: 29759078
DOI: 10.1186/s12974-018-1184-7
call_split See protocol

[…] CA, USA) producing about 500 M reads of non-paired 75-nt sequence. Up to 32 barcoded samples were pooled together producing on average 12 M reads per sample. RNAseq analysis was carried out using the BaseSpace platform from Illumina. The RNAseq-generated FASTQ files were aligned to the USCSrn5 Rattus norvegicus reference genome with STAR aligner [] with allowed mismatches set to 14. Differentially […]

library_books

Additional oxidative stress reroutes the global response of Aspergillus fumigatus to iron depletion

2018
BMC Genomics
PMCID: 5946477
PMID: 29747589
DOI: 10.1186/s12864-018-4730-x

[…] formed on an Illumina HiScan SQ instrument (Illumina, San Diego, CA, USA). Each library pool was sequenced in one lane of a sequencing flow cell, and 16–18 million reads per sample were obtained. The CASAVA software was used for pass filtering and demultiplexing processing.Reads were aligned to A fumigatus (Af293) genome (Genome: A_fumigatus_Af293_version_s03-m05-r04_chromosomes.fasta.gz;  http:// […]

library_books

Transcriptome and Co Expression Network Analyses Identify Key Genes Regulating Nitrogen Use Efficiency in Brassica juncea L.

2018
Sci Rep
PMCID: 5945678
PMID: 29748645
DOI: 10.1038/s41598-018-25826-6

[…] Paired-end (PE) read sequences of length 72 bp each with an insert-length of 260 bp were generated using CASAVA package. Quality assessment of read sequences was performed using read quality filtering tool, filteR in which poor quality reads and adapter contaminated reads were filtered-out. De novo assem […]

library_books

NOTCH signaling specifies arterial type definitive hemogenic endothelium from human pluripotent stem cells

2018
Nat Commun
PMCID: 5940870
PMID: 29739946
DOI: 10.1038/s41467-018-04134-7

[…] encing was performed using the HiSeq 3000 (Illumina, San Diego, CA) with a single read of 64 bp and index read of 7 bp.Base-calling and demultiplexing were completed with the Illumina Genome Analyzer Casava Software, version 1.8.2. Following quality assessment and filtering for adapter molecules and other sequencing artifacts, the remaining sequencing reads were aligned to transcript sequences cor […]

Citations

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BaseSpace institution(s)
Illumina, San Diego, CA, USA

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