Computational protocol: Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF induced TrkB endocytosis and dendrite outgrowth

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Protocol publication

[…] A modified Sholl analysis was performed with the Sholl analysis plugin of ImageJ (National Institutes of Health, Bethesda, MD) as described (). In brief, the center of the soma was selected as the center of all of the circles to be analyzed. The concentric circles were drawn from 10 to 100 μm by 5-μm-step intervals. The number of circles was chosen to cover the entire dendrite from soma to tips. A series of concentric circles was centered on the cell body, and the number of processes crossing each of the circles was counted. Between 30 and 35 neurons per condition were quantified, and the results at each interval were compared by an unpaired Student’s t test. […]

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