Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 15
Release date: Mar 17 2018
Last update date: Nov 30 2018
Access: Public
Diseases: DNA Virus Infections, Leukemia, Leukemia, Myeloid, Myelodysplastic Syndromes, Myeloproliferative Disorders, Neoplasms, Leukemia, Myeloid, Acute, Neoplasms, Second Primary, Hematologic Neoplasms, Leukemia, Myelomonocytic, Juvenile
Chemicals: Guanosine, Guanosine Diphosphate, Guanosine Triphosphate, Phosphates, 5-Methylcytosine
Dataset link Cooperative epigenetic remodeling by TET2 loss and NRAS mutation drives myeloid transformation and MEK inhibitor sensitivity [RNA-Seq]

Experimental Protocol


We utilized hematopoietic-tissue specific conditional Tet2/Nras double-mutant mice for our study and examined methylome of FACS-sorted LSK populations from WT, Tet2(-/-), Nras(G12D), non-leukemic Tet2(-/-) Nras(G12D), and leukemic Tet2(-/-) Nras(G12D) animals at 6 months of age. DNA was extracted using All Prep micro kit (Qiagen).

Repositories


GEO

GSE96758

ENA

SRP102075

BioProject

PRJNA379586

Download


Contact


Francine Garrett-Bakelman