Computational protocol: Collagen scaffold microenvironments modulate cell lineage commitment for differentiation of bone marrow cells into regulatory dendritic cells

Similar protocols

Protocol publication

[…] The phenotype of immature and mature DCs cultured in 2D and 3D collagen scaffolds was analysed by fluorescence-activated cell sorting (FACS Aria II, BD) after staining with the following monoclonal antibodies (mAbs), according to the manufacturer’s protocols (BD Pharmingen, Franklin Lakes, NJ): FITC-labelled anti-CD11b, APC-labelled anti-CD11c, FITC-labelled anti-CD11c, PE-labelled anti-CD40, APC-labelled anti-CD80, PE-labelled anti-CD83, PE-Cy7-labelled-anti-CD86 and PerCP-Cy5.5-labelled anti-I-A/I-E. Non-specific staining was blocked with purified rat anti-mouse CD16/CD32 (mouse BD Fc Block) (BD Pharmingen). The isotype controls were stained using the respective antibody. The expression levels of the surface markers were analysed with BD FACSDiva Software v6.1.3 and FlowJo 7.6 (FlowJo Llc, Ashland, USA). […]

Pipeline specifications

Software tools BD FACSDiva, FlowJo
Application Flow cytometry
Diseases Drug Hypersensitivity