Dataset features

Specifications


Application: ChIP-seq analysis, RNA-seq analysis
Number of samples: 441
Release date: Dec 4 2014
Last update date: Oct 24 2018
Access: Public
Dataset link Deconstructing the dynamic transcriptional program of pluripotent stem cells.

Experimental Protocol


Single-cell RNA-Seq on 183 individual v6.5 mouse embryonic stem cells (mESCs) cultured in serum+LIF media, 94 v6.5 mESCs cultured in 2i+LIF media ('ground state' conditions), and 84 Dgcr8 -/- mESCs (constructed in a v6.5 background), that lack mature miRNAs due to knockout of a miRNA processing factor, cultured in serum+LIF. ChIP-Seq for RNA polymerase II, H3K4me3, H3K27me3, H3K27ac, H3K9me3, and H3K36me3 on the three populations of mESCs profiled by single-cell RNA-Seq. Single-cell RNA-Seq on 54 individual nestin-positive neural precursor cells derived from v6.5 mESCs.

Repositories


GEO

GSE60749

ArrayExpress

E-GEOD-60749

ENA

SRP045775

BioProject

PRJNA259420

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Contact


Patrick Cahan

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