Computational protocol: Molecular insights into Adgra2/Gpr124 and Reck intracellular trafficking

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Protocol publication

[…] Images were acquired on a Zeiss LSM710 confocal microscope. Colocalization was assessed using the Pearson correlation coefficient (PCC) calculated with coloc2 in ImageJ (NIH). Applied to colocalization analysis, PCC is a measure of the linear correlation between the intensities observed in the two channels of interest. A value of 1 indicates that the two signals are positively and perfectly linearly related; a value of −1 indicates a perfectly linear, but negative correlation; and a value close to zero indicates that the distributions of the two signals are uncorrelated. [...] Statistical analyses were performed using the GraphPad Prism software. Sample size was determined with the G*power 3.1.5 software to reach adequate statistical power. Each dot plot value represents an independent cell or embryo and every experiment was conducted three times independently. P-values were calculated by the Kruskal–Wallis test (post hoc Dunn's test). […]

Pipeline specifications

Software tools Coloc, ImageJ, G*Power
Applications Miscellaneous, Laser scanning microscopy, Microscopic phenotype analysis
Organisms Danio rerio, Mus musculus
Diseases Cerebrovascular Disorders, Genetic Diseases, Inborn