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BCIP specifications

Information


Unique identifier OMICS_17337
Name BCIP
Alternative name Breast Cancer Integrative Platform
Restrictions to use None
Community driven No
Data access Browse
User data submission Not allowed
Maintained No

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Publication for Breast Cancer Integrative Platform

BCIP citations

 (346)
library_books

Secretagogin is increased in plasma from type 2 diabetes patients and potentially reflects stress and islet dysfunction

2018
PLoS One
PMCID: 5922551
PMID: 29702679
DOI: 10.1371/journal.pone.0196601

[…] shing, the Goat Anti-rabbit IgG, alkaline phosphatase labeled, secondary antibody (Pierce, Thermo Scientific) was added (1:5000) to the membrane. Immunoreactive bands were visualized using 1-step NTB/BCIP substrate (Pierce, Thermo Scientific). For detection of CHOP, fluorescent western blot with the Odyssey CLx imaging system (LI-COR biotechnology, NE, USA) was used. Proteins from the gels (10μg E […]

library_books

Species specific host factors rather than virus intrinsic virulence determine primate lentiviral pathogenicity

2018
Nat Commun
PMCID: 5893559
PMID: 29636452
DOI: 10.1038/s41467-018-03762-3

[…] were reacted with 1:1000 diluted alkaline phosphatase-conjugated goat anti-human IgG (Jackson ImmunoResearch, Europe Ltd) for 1 h followed by final color development in the dark with a one-component BCIP/NBT substrate (Moss Inc.). Initially, serum from an AGM naturally infected with SIV served as positive control, but because of weak reaction was exchanged with plasma from one of the experimental […]

library_books

Loss of XBP1 accelerates age related decline in retinal function and neurodegeneration

2018
Mol Neurodegener
PMCID: 5883257
PMID: 29615095
DOI: 10.1186/s13024-018-0250-z

[…] s washed and sections mounted with Vectashield mounting medium with DAPI (Vector, H-1200) for examination. All antibodies used are listed in Table .Cryosections of retina were incubated in 1-Step NBT/BCIP alkaline phosphatase labeling reagent (Thermo Scientific, #34070) until an appropriate chromogenic signal was observed.Photographs were taken with an Olympus DP80 digital camera mounted on an upr […]

library_books

Unravelling the Molecular Determinants of Bee Sensitivity to Neonicotinoid Insecticides

2018
Curr Biol
PMCID: 5887109
PMID: 29576476
DOI: 10.1016/j.cub.2018.02.045

[…] r at least 1 h prior to overnight incubation with the pre-adsorbed alkaline phosphatase labeled antidigoxygenin antibody (1:2000 dilution in PBS/1% BSA/0.1% Tween). The signal was visualized with NBT/BCIP alkaline phosphatase substrates according to the manufacturer’s instructions. […]

library_books

Tal1, Gata2a, and Gata3 Have Distinct Functions in the Development of V2b and Cerebrospinal Fluid Contacting KA Spinal Neurons

2018
Front Neurosci
PMCID: 5884927
PMID: 29651232
DOI: 10.3389/fnins.2018.00170

[…] riments. All confocal images were processed using Image J software (Abràmoff et al., ), in which case appropriate numbers of focal planes were merged using maximum intensity projections. For some NBT-BCIP ISH experiments, multiple planes were merged in Image J using minimum intensity projections in order to show labeled cells at different medial lateral positions in the spinal cord. All images wer […]

library_books

Resveratrol counteracts bone loss via mitofilin mediated osteogenic improvement of mesenchymal stem cells in senescence accelerated mice

2018
Theranostics
PMCID: 5928897
PMID: 29721087
DOI: 10.7150/thno.23620

[…] osphate (Sigma-Aldrich, USA) and 10 nM dexamethasone (Sigma-Aldrich, USA). The media were changed every 3 days. After induction for 7 days, alkaline phosphotase (ALP) staining was performed using the BCIP/NBT ALP color development kit (Beyotime, China) and the quantification was determined with the ImageJ 1.47 software based on grey values, while ALP activity was determined using the ALP (AKP/ALP) […]

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BCIP institution(s)
Beijing Institute of Basic Medical Sciences, Beijing, China; Department of Obstetrics and Gynecology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, China; Translational Medicine Center of Stem Cells, Laboratory of Oncology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing, China
BCIP funding source(s)
This work was supported by a grant from China National High Technology Research and Development Program 2014AA020604.

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