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BD FACSDiva specifications


Unique identifier OMICS_28164
Name BD FACSDiva
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
Computer skills Medium
Version 8.0.1
Stability Stable
Maintained No


No version available



This tool is not available anymore.

Additional information

BD FACSDiva citations


Evidence of CD4+ T cell mediated immune pressure on the Hepatitis C virus genome

Sci Rep
PMCID: 5940905
PMID: 29740042
DOI: 10.1038/s41598-018-25559-6
call_split See protocol

[…] i-PD-1-phycoerythrin (PE) (BioLegend). AquaViD (Life Technologies) was used for the exclusion of dead cells. All samples were acquired using a LSR II flow cytometer (BD Biosciences) and analyzed with BD FACSDiva software (BD Bioscience) or FlowJo v10.2 (FLOWJO). A response was deemed positive if at least three times the background. […]


Time resolved transcriptome and proteome landscape of human regulatory T cell (Treg) differentiation reveals novel regulators of FOXP3

BMC Biol
PMCID: 5937035
PMID: 29730990
DOI: 10.1186/s12915-018-0518-3

[…] ldehyde (methanol-free) in PBS instead of the FOXP3 buffer set. Samples were acquired on a FACS Canto II cytometer (BD Biosciences) and compensation was performed with the in-build compensation tool (BD FACSDiva software) using single stained samples. […]


Clinical Remission of Sight Threatening Non Infectious Uveitis Is Characterized by an Upregulation of Peripheral T Regulatory Cell Polarized Towards T bet and TIGIT

Front Immunol
PMCID: 5943505
PMID: 29774027
DOI: 10.3389/fimmu.2018.00907
call_split See protocol

[…] ence-minus-one (FMO) controls for CD25, TIGIT, FoxP3, RORγt, and T-bet were included in each experiment.Flow cytometric data were acquired using an LSRFortessa (BD Biosciences, San Jose, CA, USA) and BD FACSDiva version 6.1.3 software with a total of 50,000 events being recorded for each sample through a live, single-cell CD3+CD4+ gate (gating strategy shown in Figure ). Viable cells were identifi […]


Initial characterisation of adult human ovarian cell populations isolated by DDX4 expression and aldehyde dehydrogenase activity

Sci Rep
PMCID: 5934371
PMID: 29725036
DOI: 10.1038/s41598-018-25116-1

[…] allow subsequent gating. DDX4-positive and negative cells, for both human ovarian cells and transfected HEK 293 T cells, were sorted using an Aria IIu cytometer equipped with a 488 nm laser, running BD FACSDiVa v6 software (BD Biosciences, Oxford, UK). Cells were sorted on high purity setting at no more than 5000 events/s using a 70 µm nozzle at 55 psi with a 90 Hz droplet stream. Where collectio […]


Tumor cure by radiation therapy and checkpoint inhibitors depends on pre existing immunity

Sci Rep
PMCID: 5934473
PMID: 29725089
DOI: 10.1038/s41598-018-25482-w

[…] iences) and frozen at −80 °C. For analysis cells were thawed and intracellularly stained with IFNγ-APC. Cells were washed and analyzed on a BD LSRII Flow Cytometer and the data was interrogated using BD FACSDiva (BD Biosciences) and FloJo (Tree Star, Ashland, OR). […]


Podosomes, But Not the Maturation Status, Determine the Protease Dependent 3D Migration in Human Dendritic Cells

Front Immunol
PMCID: 5936769
PMID: 29760696
DOI: 10.3389/fimmu.2018.00846
call_split See protocol

[…] staining, the cells were washed with cold staining buffer, centrifuged twice for 5 min at 340 g at 4°C, and analyzed by flow cytometry using LSR-II flow cytometer (BD Biosciences) and the associated BD FACSDiva software. Data were then analyzed using the FlowJo 7.6.5 software (TreeStar). […]


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