Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Aug 11 2017
Last update date: Jan 24 2018
Access: Public
Diseases: Arthritis, Arthritis, Experimental, Arthritis, Rheumatoid, Autoimmune Diseases
Chemicals: Methotrexate
Dataset link Ras signaling inhibitors in in Adjuvant-induced Arthritis via targeting pathogenic antigen-specific Th17-type cells

Experimental Protocol


Eight-week-old Lewis rats will be injected at the base of the tail with 100 μl CFA prepared by suspending heat-killed M. tuberculosis (Difco) in mineral oil at 10 mg/ml. For determining the clinical outcomes, we will measure starting day +9 the ankle diameter and score all paws on a scale of 0–4 for degree of swelling, redness, and deformity of the joints. For drug therapy, we will randomly divide the rats into two groups. Rats in the first group will be treated daily (starting on day +1 after AIA induction) with oral FTS (100 mg/kg). Rats in the second group of control Rats will receive only the oral vehicle CMC control (0.5% carboxymethyl cellulose). At experiment end, the will be sacrificed and their spleens will be harvested for further testing. Single cell suspensions of spleen will be cultures with HSP65 (AG stimulation) or control media (Med group) for 72 hrs. Immediately thereafter, we will be process the cells for RNA isolation. Total RNA will be extracted and purified using the Direct-zol™ RNA MiniPrep w/ TRI-Reagent kits (Zymo Research Corp.) N= 8 samples ( 2 samples CMC Med, 2 samples CMC AG, 2 samples FTS Med, 2 samples FTS AG

Repositories


GEO

GSE100280

BioProject

PRJNA391245

Download


Contact


jasmine jacob

Dataset Statistics

info

Citations per year

Dataset publication