Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 8
Release date: Apr 25 2014
Last update date: Jul 27 2018
Access: Public
Diseases: Parasitic Diseases
Dataset link microRNA expression profile in Leishmania donovani infected mouse macrophage

Experimental Protocol


Peritoneal exudate macrophages were isolated from mouse, grown in 60mm plates and infected with Leishmania donovani and total RNA was isolated from cells at 12, 18 and 24 hrs post infection. Leishmania infected macrophage miRNA expression signature was generated. Cells grown on 60mm plates and infected with Leishmania. The main objective of the microarray analysis of mmu-miRNA in antimony resistant and antimony sensitive Leishmania donovani infected macrophages are as follows: 1. To study how the expression of miRNA varies in either antimony resistant or antimony sensitive Leishmania infected macrophages as compared to the normal macrophages as a function of time. LPS was used as control. 2. To study the expression of those miRNAs which are differentially expressed in antimony resistant and antimony sensitive Leishmania infected macrophages at each time point post infection. 3. To identify those miRNAs which are responsible for degradation of autophagy initiating protein beclin 1 mRNA

Repositories


GEO

GSE57042

ArrayExpress

E-GEOD-57042

BioProject

PRJNA245394

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Contact


Syamal Roy