Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Apr 25 2011
Last update date: Nov 6 2013
Access: Public
Diseases: Testicular Neoplasms
Dataset link Basic helix-loop-helix transcription factor Tcf21 is downstream target of male sex-determining gene SRY

Experimental Protocol


We used microarrays to determine genes whose expression was stimulated in rat E13 ovarian cell sub-cultures in the presence of a pCMV-myc-expression plasmid over-expressing the Sry, Tsf21, and/or Tcf12 (Reb-alfa) genes. RNA samples from the control group (untreated E13 rat ovarian cells) are compared to RNA from 4 groups of treated E13 rat ovarian cells: 1) Sry overexpressing, 2) Tcf21 overexpressing, 3) Tcf12 (Reb alfa) overexpressing, and 4) Tcf21 + Tcf12 (Reb-alfa) overexpressing. Untreated E13 testis cell sub-cultures were also analyzed. 3 biological replicates each group.

Repositories


GEO

GSE24666

ArrayExpress

E-GEOD-24666

BioProject

PRJNA132539

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Contact


Michael Skinner
Michael K Skinner

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