Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 54
Release date: Nov 13 2015
Last update date: Dec 3 2015
Access: Public
Dataset link Host-virus transcriptome dynamics determined from RNA-sequencing

Experimental Protocol


We investigated the transcriptional program of the Syn9 cyanophage during infection of three Synechococcus hosts (strains WH7803, WH8102 and WH8109) at different times after infection using whole transcriptome RNA sequence libraries. Four time points (5 min and 0.5, 1 and 2 h after infection) from two biological replicates of each of the three hosts were analyzed for a total of 24 samples. Pooled replicates of one of the infected Synechococcus hosts from 3 time points (0.5, 1 and 2 h after infection) were used for bisulfite strand-specific libraries (for a total of 3 samples). For 5?-end sequencing, Tobacco Acid Pyrophosphatase (TAP) treated libraries were prepared from two biological replicates for time points 0.5, 1 and 2 h after infection for a total of 18 samples. Untreated libraries were similarly prepared for one replicate of each infected host for a total of 9 libraries. All RNA-sequencing libraries were sequenced using the Illumina HiSeq platform at the Weizmann Institute.

Repositories


GEO

GSE74921

ENA

SRP066156

BioProject

PRJNA302091

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Contact


Shany Doron