Dataset features


Application: miRNA array analysis
Number of samples: 3
Release date: Jan 13 2018
Last update date: Jan 24 2018
Access: Public
Dataset link Expression data from purified mouse CD4+ T cells

Experimental Protocol

Female C57BL/6 mice were given either tail-skin syngeneic grafts (C57BL/6 --> C57BL/6) or allogenic grafts (C3H --> C57BL/6) on the dorsal lateral surface of the recipient mouse. Allograft directed inflammation or syngeneic graft acceptance proceeded for 10 days. Mice were sacrificed and their draining lymph nodes (axillary and brachial) were harvested. CD4+ T cells were purified from single cell suspensions of this population via magnetic bead isolation. Purity was verified to be > 90% CD4+ by flow cytometry before the purified cells were subjected to total RNA extraction before hybridization on Affymetrix arrays. Naive CD4+ T cells from axillary and brachial lymph nodes were extracted and purified as well for a non-surgery control. Thus, samples were from three groups: Naive CD4+ T cells (N4), allograft-responding CD4+ cells (Al4) or syngeneic graft-responding CD4+ cells (Au4).








William Becker