Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 36
Release date: Jul 1 2013
Last update date: Mar 20 2017
Access: Public
Diseases: Melanoma, Tumor Lysis Syndrome
Chemicals: Cisplatin
Dataset link Cisplatin treated melanoma and melanocyte cell lines

Experimental Protocol


One melanocyte, three primary melanoma (MM200, IgR3, Me4405) and two metastatic melanoma (Mel-RM and Sk-mel-28) cell lines were treated with cispltain and gene expression profile data collected at 0, 6 and 24 hours. Biological duplicates were treated and RNA was collected for each cell line at each timepoint. The duplicated were run sperately on the WGGEX beadarrays and the results of the duplicates averaged for publication. The transcript expression results were cubic spline normalised using BeadStudio 2.0 software (Illumina, USA), and the remaining analyses was performed using GeneSpring GX 11.0. To account for bias or skewing of expression results all the gene expression profiles and each individual gene were normalized to the median resulting in two way normalisation. For visualisation of the results the data was log transformed.

Repositories


GEO

GSE47980

ArrayExpress

E-GEOD-47980

BioProject

PRJNA208525

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Contact


Nikola Bowden

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