Computational protocol: The N-terminus of mature human frataxin is intrinsically unfolded

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Protocol publication

[…] Eukaryotic frataxin sequences were retrieved by a BLASTp search (http://blast.ncbi.nlm.nih.gov) [] and aligned using Clustal X version 2.0 (http://www.clustal.org) []. Intrinsically unfolded regions were predicted using the DISEMBL (http://dis.embl.de/) [] and PONDR (http://www.pondr.com/) [] web servers. Chemical shift analysis was carried out using the CSI (chemical shift indices) (http://www.bionmr.ualberta.ca/bds/software/csi/latest/csi.html) [] and RCI (random coil index) (http://wishart.biology.ualberta.ca/rci/cgi-bin/rci_cgi_1_e.py) [] web servers. […]

Pipeline specifications

Software tools BLASTP, Clustal W, DisEMBL, RCI
Application Protein structure analysis
Organisms Homo sapiens
Diseases Friedreich Ataxia, Heredodegenerative Disorders, Nervous System
Chemicals Iron