Dataset features

Specifications


Application: ChIP-seq analysis, RNA-seq analysis
Number of samples: 48
Release date: Jul 18 2016
Last update date: Nov 21 2018
Access: Public
Dataset link Chromatin Remodeling Inactivates Activity Genes and Regulates Neural Coding

Experimental Protocol


One or two replicates of the histone modifications (H3K27me3 and H2A.z), total histone proteins (H2A.z and H3), and ATPase Chd4 using postnatal day 22 cerebella from wild type (WT) or Chd4 conditional knockout (cKO) mice were examined using libraries prepared with the Illumina ChIP-Seq DNA Sample Prep Kit. Four replicates of total RNA were extracted from postnatal day 27-28 cerebella from rotarod-trained or control homecage mice, or Chd4 cKO or WT mice using Trizol and reverse-transcribed with oligo-dT priming. Three replicates of immunoprecipitated Sync-TRAP RNA or the input control using postnatal day 12 Chd4 cKO or WT cerebella were purified and amplified with Ovation RNA-Seq System V2 (NuGEN). All samples were sequenced on the Illumina HiSeq 2000 platform.

Repositories


GEO

GSE83253

ArrayExpress

E-GEOD-83253

ENA

SRP076455

BioProject

PRJNA325442

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Contact


Yue Yang