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Protocols

Beacon Designer specifications

Information


Unique identifier OMICS_15903
Name Beacon Designer
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Mac OS, Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes
Wikipedia https://en.wikipedia.org/wiki/Beacon_designer

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No version available

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  • person_outline Beacon Designer Team

Beacon Designer citations

 (1047)
library_books

Neuro inflammatory effects of photodegradative products of bilirubin

2018
Sci Rep
PMCID: 5945592
PMID: 29748620
DOI: 10.1038/s41598-018-25684-2

[…] ed with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). For the quantitative RT-PCR in organotypic rat hippocampal slices, primers were designed using the Beacon designer 4.2 software (Premier Biosoft International, Palo Alto, CA, USA) on rat sequences available in GenBank (Table ). The reaction was performed in a final volume of 15 μL in an iQ5 Bio-Rad […]

library_books

Proteomic analysis of watery saliva secreted by white backed planthopper, Sogatella furcifera

2018
PLoS One
PMCID: 5935387
PMID: 29727440
DOI: 10.1371/journal.pone.0193831

[…] rlsbad, CA, USA). Total RNA of the whole insect was extracted by using TRIzol Reagent (Invitrogen).Gene-specific primers () were designed using primer3 web (version 4.0.0) (http://primer3.ut.ee/) and Beacon Designer 7.90, and the cDNA was prepared according to the instruction. The S. furcifera housekeeping gene Ribosomal protein L9 (GeneBank accession number KP735523) and Ribosomal protein L10 (Ge […]

library_books

Identification of Chemosensory Genes Based on the Transcriptomic Analysis of Six Different Chemosensory Organs in Spodoptera exigua

2018
Front Physiol
PMCID: 5928209
PMID: 29740343
DOI: 10.3389/fphys.2018.00432

[…] H, Nanjing, China) as the manufacturer's instructions. Briefly, the reaction programs were 10 min at 95°C, 40 cycles of 95°C for 15 s and 60°C for 1 min. The qPCR primers (Table ) were designed using Beacon Designer 7.9 (PREMIER Biosoft International, CA, USA). Then, the relative expression levels of SexiGRs mRNA were calculated based on the Ct-values of target gene and two reference genes SexiGAP […]

library_books

Identification of microRNAs associated with the exogenous spermidine mediated improvement of high temperature tolerance in cucumber seedlings (Cucumis sativus L.)

2018
BMC Genomics
PMCID: 5937831
PMID: 29690862
DOI: 10.1186/s12864-018-4678-x

[…] the Step One ™ Real-time PCR System (Applied Biosystems, Singapore) using the Super Real Fluorescence Quantification Kit (SYBR Green) (FP205, Tiangen, China). The target gene primers were designed by Beacon Designer 7.9. The cucumber actin gene was used as an internal reference to normalize the qRT-PCR data.All reactions were carried out according to the manufacturer’s instructions and repeated th […]

call_split

Genome Wide Identification, Evolutionary, and Expression Analysis of VQ Genes from Two Pyrus Species

2018
Genes
PMCID: 5924566
PMID: 29690608
DOI: 10.3390/genes9040224
call_split See protocol

[…] rt Tip Green qPCR SuperMix (TransGen Biotech, Beijing, China). According to a previous workflow, the normalization of each reaction threshold cycle (Ct) value was determined by pear tubulin gene [,]. Beacon Designer v7.9 was used to design specific primers of the qRT-PCR analysis [,], which are presented in . All expression data were obtained from three biological repeats. The SPSS v19.0 software […]

library_books

Anti Diabetic Effects of Dung Beetle Glycosaminoglycan on db Mice and Gene Expression Profiling

2018
PMCID: 5903143
PMID: 29686777
DOI: 10.5487/TR.2018.34.2.151

[…] pplied Biosystems, Fostercity, CA, USA), according to the manufacturer’s instructions. For detection of target gene transcripts, we designed specific forward and reverse oligonucleotide primers using Beacon Designer software (PREMIER Biosoft, Palo Alto, CA, USA). The primer sequences are listed in . Target mRNA levels were normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as an int […]


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Beacon Designer institution(s)
PREMIER Biosoft, CA, USA

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