Beacon Designer specifications


Unique identifier OMICS_15903
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Mac OS, Windows
Computer skills Medium
Stability Stable
Maintained Yes



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Beacon Designer citations

PMCID: 5928209

[…] nanjing, china) as the manufacturer's instructions. briefly, the reaction programs were 10 min at 95°c, 40 cycles of 95°c for 15 s and 60°c for 1 min. the qpcr primers (table s2) were designed using beacon designer 7.9 (premier biosoft international, ca, usa). then, the relative expression levels of sexigrs mrna were calculated based on the ct-values of target gene and two reference genes […]

PMCID: 5247736

[…] gse74544., for verification of transcriptome data, qpcr was performed by lightcycler 480 system (roche, germany) with 2 × sybr premix ex taq (takara, dalian, china). the primers were designed by beacon designer 7 and the sequences were listed in supplementary table s1., as the major genes of central metabolism were identified by transcriptome, these genes’ expression levels of h915-1 at 48 h […]

PMCID: 4958654

[…] rt kits (takara, japan) according to the manufacturer’s instructions, and a fivefold dilution of the resulting cdna was used as the template for qrt-pcr analysis. primer sequences (designed by beacon designer 7) were listed in supplementary table s1. qrt-pcr was performed on a real-time pcr instrument (analytik jena ag, germany). the reaction mixture (20 μl total volume) contained 10 μl […]

PMCID: 4936700

[…] (invitrogen). the specific stem-loop primers and forward primers for the mirnas were designed based on the mature mirna sequences [52], and the primers for their target mrnas were designed using beacon designer, version 7.7 (premier biosoft international, ltd., palo alto, ca, usa). the u6 snrna and 18s rrna of paulownia were used as the reference genes for the mirnas and targets, […]

PMCID: 4140679

[…] scientific inc., waltham, ma, usa) with a faststart universal syber green master mix (roche diagnostics., indianapolis, usa) according to manufacturer's instructions. pcr primers were designed using beacon designer 7 software ( eight-fold serial dilutions of the plasmids from peasy-t1 (beijing transgen biotech co., ltd, beijing, china) […]

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