Beacon Designer pipeline

Beacon Designer specifications

Information


Unique identifier OMICS_15903
Name Beacon Designer
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Mac OS, Windows
Computer skills Medium
Stability Stable
Maintained Yes
Wikipedia https://en.wikipedia.org/wiki/Beacon_designer

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Beacon Designer IN pipelines

 (11)
2017
PMCID: 5247736
PMID: 28106122
DOI: 10.1038/srep41040

[…] gse74544., for verification of transcriptome data, qpcr was performed by lightcycler 480 system (roche, germany) with 2 × sybr premix ex taq (takara, dalian, china). the primers were designed by beacon designer 7 and the sequences were listed in supplementary table s1., as the major genes of central metabolism were identified by transcriptome, these genes’ expression levels of h915-1 at 48 h […]

2016
PMCID: 4734323
PMID: 26829252
DOI: 10.1038/srep20368

[…] sequenced. quantitative real-time pcr (qpcr) was performed using the mastercycler ep realplex system (eppendorf, hamburg, germany). gene-specific primers (supplementary table s1) were designed using beacon designer 7.6 and synthesized by genewiz co. ltd. (beijing, china). dimethyladenosine transferase (genbank: kf018923) and peptidyl-prolyl cis-trans isomerase (genbank: kf018924) were used […]

2016
PMCID: 4936700
PMID: 27388154
DOI: 10.1371/journal.pone.0158750

[…] (invitrogen). the specific stem-loop primers and forward primers for the mirnas were designed based on the mature mirna sequences [52], and the primers for their target mrnas were designed using beacon designer, version 7.7 (premier biosoft international, ltd., palo alto, ca, usa). the u6 snrna and 18s rrna of paulownia were used as the reference genes for the mirnas and targets, […]

2016
PMCID: 4958654
PMID: 27504115
DOI: 10.3389/fpls.2016.01094

[…] rt kits (takara, japan) according to the manufacturer’s instructions, and a fivefold dilution of the resulting cdna was used as the template for qrt-pcr analysis. primer sequences (designed by beacon designer 7) were listed in supplementary table s1. qrt-pcr was performed on a real-time pcr instrument (analytik jena ag, germany). the reaction mixture (20 μl total volume) contained 10 μl […]

2015
PMCID: 4366804
PMID: 25792497
DOI: 10.1038/srep09324

[…] system (promega) following the instructions in the manual., specific primers for the transcripts putatively involved in pheromone biosynthesis, reception, and degradation were designed using beacon designer 7.7 (premier biosoft, palo alto, ca, usa) (supplementary table s6 online). semi-quantitative pcr experiments with negative controls (no cdna template) were performed as follows: 94°c […]

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